cover lips  (Valiant Co Ltd)

Journal: Cancer Science

Article Title: C/EBPβ induces B‐cell acute lymphoblastic leukemia and cooperates with BLNK mutations

doi: 10.1111/cas.15164

Figure Lengend Snippet: Transgenic expression of the Cebpb LAP isoform accelerated pre‐B‐ALL development in Blnk homozygous KO mice. A, Kaplan‐Meier survival curve for mice of indicated genotypes. Giemsa staining. B, Representative morphologies of pre‐B‐ALL developed in LAP transgenic, Blnk homozygous KO and LAP/Blnk homozygous KO mice, and B lymphoblastic leukemia in LAP transgenic mice. C, Surface marker expression in ALL of indicated genotypes. D, Distribution of disease types developed in LAP transgenic, Blnk homozygous KO, and LAP/Blnk homozygous KO mice. E, Principal component analysis for gene expression profiles in leukemia of each genotype. F, GSEA of LAP/Blnk homozygous KO vs LAP transgenic leukemia shows enrichment of gene sets involved in the AKT pathway

Article Snippet: To generate Cebpb transgenic mice, Cebpb cDNA, covering the entire coding regions of either LAP or LIP , were amplified using PCR and subcloned into the p3xFLAG‐CMV‐10 vector (Sigma).

Techniques: Transgenic Assay, Expressing, Staining, Marker

Journal: Cancer Science

Article Title: C/EBPβ induces B‐cell acute lymphoblastic leukemia and cooperates with BLNK mutations

doi: 10.1111/cas.15164

Figure Lengend Snippet: Transgenic expression of the Cebpb LAP isoform enhances differentiation block in Blnk homozygous KO mice. A, The number of B220‐positive and IgM‐high mature B‐cells in bone marrow was measured in mice of 6 wk of age for the indicated genotype. Statistical significance was assessed using Scheffé test (** P < .01, *** P < .001). B, Representative results of flow cytometric analysis. Frequencies of B220‐ and IL‐7Rα‐positive B‐cells are indicated. C, The number of B220‐ and IL‐7Rα‐positive B‐cells in the bone marrow was measured in mice of 6 wk of age for the indicated genotype. Statistical significance was assessed using Scheffé test (*** P < .001). D, Differential arrest at the pre‐BI stage (B220 high CD43 high CD24 intermediate ) of KO and LAP + KO mice. E, Immunoblotting shows increased phosphorylation of STAT5 in bone marrow B‐cells. F, PCA of all groups of bone marrow cells. G, GSEA of bone marrow B‐cells from LAP/Blnk homozygous KO shows enrichment of gene pathways involved in pre‐BI lymphocyte differentiation (left) and hematopoietic stem cells (right)

Article Snippet: To generate Cebpb transgenic mice, Cebpb cDNA, covering the entire coding regions of either LAP or LIP , were amplified using PCR and subcloned into the p3xFLAG‐CMV‐10 vector (Sigma).

Techniques: Transgenic Assay, Expressing, Blocking Assay, Western Blot